Expanded breadth of the T-cell response to mosaic human immunodeficiency virus type 1 envelope DNA vaccination.
Identifieur interne : 002795 ( Main/Exploration ); précédent : 002794; suivant : 002796Expanded breadth of the T-cell response to mosaic human immunodeficiency virus type 1 envelope DNA vaccination.
Auteurs : Wing-Pui Kong [États-Unis] ; Lan Wu ; Timothy C. Wallstrom ; Will Fischer ; Zhi-Yong Yang ; Sung-Youl Ko ; Norman L. Letvin ; Barton F. Haynes ; Beatrice H. Hahn ; Bette Korber ; Gary J. NabelSource :
- Journal of virology [ 1098-5514 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Déterminants antigéniques des lymphocytes T (immunologie), Femelle, Immunité cellulaire, Lymphocytes T (immunologie), Lymphocytes T (virologie), Modèles statistiques, Produits du gène env du virus de l'immunodéficience humaine (immunologie), Souris, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), Vaccins contre le SIDA (immunologie), Vaccins à ADN (immunologie).
- MESH :
- immunologie : Déterminants antigéniques des lymphocytes T, Lymphocytes T, Produits du gène env du virus de l'immunodéficience humaine, VIH-1 (Virus de l'Immunodéficience Humaine de type 1), Vaccins contre le SIDA, Vaccins à ADN.
- virologie : Lymphocytes T.
- Animaux, Femelle, Immunité cellulaire, Modèles statistiques, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : AIDS Vaccines, Epitopes, T-Lymphocyte, Vaccines, DNA, env Gene Products, Human Immunodeficiency Virus.
- immunology : HIV-1, T-Lymphocytes.
- virology : T-Lymphocytes.
- Animals, Female, Immunity, Cellular, Mice, Models, Statistical.
Abstract
An effective AIDS vaccine must control highly diverse circulating strains of human immunodeficiency virus type 1 (HIV-1). Among HIV-1 gene products, the envelope (Env) protein contains variable as well as conserved regions. In this report, an informatic approach to the design of T-cell vaccines directed to HIV-1 Env M group global sequences was tested. Synthetic Env antigens were designed to express mosaics that maximize the inclusion of common potential T-cell epitope (PTE) 9-mers and minimize the inclusion of rare epitopes likely to elicit strain-specific responses. DNA vaccines were evaluated using intracellular cytokine staining in inbred mice with a standardized panel of highly conserved 15-mer PTE peptides. One-, two-, and three-mosaic sets that increased theoretical epitope coverage were developed. The breadth and magnitude of T-cell immunity stimulated by these vaccines were compared to those for natural strain Envs; additional comparisons were performed on mutant Envs, including gp160 or gp145 with or without V regions and gp41 deletions. Among them, the two- or three-mosaic Env sets elicited the optimal CD4 and CD8 responses. These responses were most evident in CD8 T cells; the three-mosaic set elicited responses to an average of eight peptide pools, compared to two pools for a set of three natural Envs. Synthetic mosaic HIV-1 antigens can therefore induce T-cell responses with expanded breadth and may facilitate the development of effective T-cell-based HIV-1 vaccines.
DOI: 10.1128/JVI.02256-08
PubMed: 19109395
Affiliations:
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Le document en format XML
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Wallstrom</name></author><author><name sortKey="Fischer, Will" sort="Fischer, Will" uniqKey="Fischer W" first="Will" last="Fischer">Will Fischer</name></author><author><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name></author><author><name sortKey="Ko, Sung Youl" sort="Ko, Sung Youl" uniqKey="Ko S" first="Sung-Youl" last="Ko">Sung-Youl Ko</name></author><author><name sortKey="Letvin, Norman L" sort="Letvin, Norman L" uniqKey="Letvin N" first="Norman L" last="Letvin">Norman L. Letvin</name></author><author><name sortKey="Haynes, Barton F" sort="Haynes, Barton F" uniqKey="Haynes B" first="Barton F" last="Haynes">Barton F. Haynes</name></author><author><name sortKey="Hahn, Beatrice H" sort="Hahn, Beatrice H" uniqKey="Hahn B" first="Beatrice H" last="Hahn">Beatrice H. Hahn</name></author><author><name sortKey="Korber, Bette" sort="Korber, Bette" uniqKey="Korber B" first="Bette" last="Korber">Bette Korber</name></author><author><name sortKey="Nabel, Gary J" sort="Nabel, Gary J" uniqKey="Nabel G" first="Gary J" last="Nabel">Gary J. Nabel</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS Vaccines (immunology)</term><term>Animals</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Female</term><term>HIV-1 (immunology)</term><term>Immunity, Cellular</term><term>Mice</term><term>Models, Statistical</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes (virology)</term><term>Vaccines, DNA (immunology)</term><term>env Gene Products, Human Immunodeficiency Virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Femelle</term><term>Immunité cellulaire</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T (virologie)</term><term>Modèles statistiques</term><term>Produits du gène env du virus de l'immunodéficience humaine (immunologie)</term><term>Souris</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie)</term><term>Vaccins contre le SIDA (immunologie)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>AIDS Vaccines</term><term>Epitopes, T-Lymphocyte</term><term>Vaccines, DNA</term><term>env Gene Products, Human Immunodeficiency Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Déterminants antigéniques des lymphocytes T</term><term>Lymphocytes T</term><term>Produits du gène env du virus de l'immunodéficience humaine</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>Vaccins contre le SIDA</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>HIV-1</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Lymphocytes T</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Immunity, Cellular</term><term>Mice</term><term>Models, Statistical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Immunité cellulaire</term><term>Modèles statistiques</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An effective AIDS vaccine must control highly diverse circulating strains of human immunodeficiency virus type 1 (HIV-1). Among HIV-1 gene products, the envelope (Env) protein contains variable as well as conserved regions. In this report, an informatic approach to the design of T-cell vaccines directed to HIV-1 Env M group global sequences was tested. Synthetic Env antigens were designed to express mosaics that maximize the inclusion of common potential T-cell epitope (PTE) 9-mers and minimize the inclusion of rare epitopes likely to elicit strain-specific responses. DNA vaccines were evaluated using intracellular cytokine staining in inbred mice with a standardized panel of highly conserved 15-mer PTE peptides. One-, two-, and three-mosaic sets that increased theoretical epitope coverage were developed. The breadth and magnitude of T-cell immunity stimulated by these vaccines were compared to those for natural strain Envs; additional comparisons were performed on mutant Envs, including gp160 or gp145 with or without V regions and gp41 deletions. Among them, the two- or three-mosaic Env sets elicited the optimal CD4 and CD8 responses. These responses were most evident in CD8 T cells; the three-mosaic set elicited responses to an average of eight peptide pools, compared to two pools for a set of three natural Envs. Synthetic mosaic HIV-1 antigens can therefore induce T-cell responses with expanded breadth and may facilitate the development of effective T-cell-based HIV-1 vaccines.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Fischer, Will" sort="Fischer, Will" uniqKey="Fischer W" first="Will" last="Fischer">Will Fischer</name><name sortKey="Hahn, Beatrice H" sort="Hahn, Beatrice H" uniqKey="Hahn B" first="Beatrice H" last="Hahn">Beatrice H. Hahn</name><name sortKey="Haynes, Barton F" sort="Haynes, Barton F" uniqKey="Haynes B" first="Barton F" last="Haynes">Barton F. Haynes</name><name sortKey="Ko, Sung Youl" sort="Ko, Sung Youl" uniqKey="Ko S" first="Sung-Youl" last="Ko">Sung-Youl Ko</name><name sortKey="Korber, Bette" sort="Korber, Bette" uniqKey="Korber B" first="Bette" last="Korber">Bette Korber</name><name sortKey="Letvin, Norman L" sort="Letvin, Norman L" uniqKey="Letvin N" first="Norman L" last="Letvin">Norman L. Letvin</name><name sortKey="Nabel, Gary J" sort="Nabel, Gary J" uniqKey="Nabel G" first="Gary J" last="Nabel">Gary J. Nabel</name><name sortKey="Wallstrom, Timothy C" sort="Wallstrom, Timothy C" uniqKey="Wallstrom T" first="Timothy C" last="Wallstrom">Timothy C. Wallstrom</name><name sortKey="Wu, Lan" sort="Wu, Lan" uniqKey="Wu L" first="Lan" last="Wu">Lan Wu</name><name sortKey="Yang, Zhi Yong" sort="Yang, Zhi Yong" uniqKey="Yang Z" first="Zhi-Yong" last="Yang">Zhi-Yong Yang</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Kong, Wing Pui" sort="Kong, Wing Pui" uniqKey="Kong W" first="Wing-Pui" last="Kong">Wing-Pui Kong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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